1 – What is SPR?
1.1 – TIR – Total Internal Reflection
1.2 – SPR – Surface Plasmon Resonance
1.3 – SPR Biosensor
1.4 – SPR Language
2 – Tips on Sample Preparation and Experimental Setup
2.1 – Usage of Running- and Sample wash buffer
2.2 – Tips on the sample preparation
2.3 – Tips on experimental setup
3 – Sensor surfaces
3.1 – General build-up
3.2 – Bruker sensor surfaces
4 – Precondition and Preconcentration
4.1 – Precondition
4.2 – Preconcentration
5 – Immobilization
5.1 – Direct coupling
5-2 Indirect coupling / capture
5.3 – Direct vs. indirect
5.4 – Immobilization levels
6 – Antibody Interaction
6.1 Recommended setup
6.2 Experimental hints
7 – Small molecule Interaction
7.1 – General Aspects
7.2 – Experimental Hints
8 – Maintenance
8.1 – Parts to be cleaned
8.2 – Cleaning routines
8.3 – Functional Test
Here you find some basic knowledge about SPR (Surface Plasmon Resonance) in general and some more specific information about realtime label free analysis.
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Table of Contents
1 – What is SPR?
1.1 – TIR – Total Internal Reflection
1.2 – SPR – Surface Plasmon Resonance
1.3 – SPR Biosensor
1.4 – SPR Language
2 – Tips on Sample Preparation and Experimental Setup
2.1 – Usage of Running- and Sample wash buffer
2.2 – Tips on the sample preparation
2.3 – Tips on experimental setup
3 – Sensor surfaces
3.1 – General build-up
3.2 – Bruker sensor surfaces
4 – Precondition and Preconcentration
4.1 – Precondition
4.2 – Preconcentration
5 – Immobilization
5.1 – Direct coupling
5-2 Indirect coupling / capture
5.3 – Direct vs. indirect
5.4 – Immobilization levels
6 – Antibody Interaction
6.1 Recommended setup
6.2 Experimental hints
7 – Small molecule Interaction
7.1 – General Aspects
7.2 – Experimental Hints
8 – Maintenance
8.1 – Parts to be cleaned
8.2 – Cleaning routines
8.3 – Functional Test
Continue to 1 - What is SPR?