3 - Sensor Surfaces

3.1 General build-up

Figure 3-1: Schematic illustration of a SPR sensor chip. The glass slide or prism is coated with an 1- 2 nm adhesive layer carrying the 48-52 nm of gold. Additionally, a certain surface chemistry is used to allow capturing of biomolecules.

3.2 Bruker sensor surfaces

  • self-assembled monolayer with exposed COOH, 2D surface
  • low immobilization levels; ~ 10-20% of the HCA immob level
  • Large molecules, e.g. VLP, cells
Order Number: 1862611 / 1862612
  • carboxymethylated dextran matrix exposing multiple COOH-groups, 3D surface
  • medium – high immobilization levels; coupling via NH2, CHO, COOH, SH
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids
Order Number: 1862614 / 1862615
  • carboxymethylated dextran matrix with pre-immobilized amine-NTA, 3D surface
  • medium – high immobilization levels; coupling via His-Tag
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids
Order Number: 1862617 / 1862618
  • carboxymethylated dextran matrix with pre-immobilized Neutravidin, 3D surface
  • medium – high immobilization levels; coupling via Biotin-Tag
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids
Order Number: 1862620 / 1862621
  • carboxymethylated dextran matrix with pre-immobilized Protein A/G, 3D surface
  • medium – high immobilization levels;
  • most subclasses of e.g. human-, mouse-, rat-, horse-IgG
Order Number: 1862623 / 1862624
  • Contains a self-assembled monolayer with exposed CH3, 2D surface
  • No detergents in running buffer as capture is mostly hydrophobic
  • Large molecules, e.g. membranes, cells
Order Number: 1862626 / 1862627
Create your own surface with e.g. alkanthiols.

Order Number: 1862632 / 1862633
Continue to 4 - Precondition and Preconcentration