3 - Sensor Surfaces

3.1 General build-up

Figure 3-1: Schematic illustration of a SPR sensor chip. The glass slide or prism is coated with an 1- 2 nm adhesive layer carrying the 48-52 nm of gold. Additionally, a certain surface chemistry is used to allow capturing of biomolecules.

3.2 Bruker sensor surfaces

  • self-assembled monolayer with exposed COOH, 2D surface
  • low immobilization levels; ~ 10-20% of the HCA immob level
  • Large molecules, e.g. VLP, cells

Order Number: 1814894

  • carboxymethylated dextran matrix exposing multiple
    COOH-groups, 3D surface
  • medium – high immobilization levels; coupling via NH2,
    CHO, COOH, SH
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids

Order Number: 1814895 (single sensor)

  • carboxymethylated dextran matrix with pre-immobilized amine-NTA, 3D surface
  • medium – high immobilization levels; coupling via His-Tag
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids

Order Number: 1814896

  • carboxymethylated dextran matrix with pre-immobilized Neutravidin, 3D surface
  • medium – high immobilization levels; coupling via Biotin-Tag
  • All types of biomolecules, e.g. lipids, proteins, nucleic acids

Order Number: 1862619

  • carboxymethylated dextran matrix with pre-immobilized Protein A/G, 3D surface
  • medium – high immobilization levels;
  • most subclasses of e.g. human-, mouse-, rat-, horse-IgG

Order Number: 1814899

All sensors and further details on their surface and the corresponding product sheets can be found in the webshop of Bruker:
https://store.bruker.com/components/surface-plasmon-resonance